Ph.D. Plant Pathology, Oklahoma State University, 2001
Erwinia amylovora gene regulatory networks (GRNs) - Fire blight, caused by Erwinia amylovora, is a particularly devastating disease for the apple and pear fruit industry. Our long-term goal is to comprehensively understand the regulation of virulence factors at both transcriptional and post-transcriptional levels, and to reconstruct gene regulatory networks (GRNs) in E. amylovora, thus allowing a better understanding of the basic mechanisms of pathogenicity and biology of E. amylovora.
From genome to control of fire blight - Type III secretion system (T3SS) is a universal target for developing novel antibacterial agents -Recent advances in studying bacterial virulence factors provide mounting evidence that, the T3SS system is a potent virulence mechanism shared by a broad spectrum of pathogenic Gram negative bacteria that infect both plant and mammalian hosts by injecting effector proteins into host cells. Thus, the T3SS apparatus is essential for bacteria to evade the host immune defense. Our long-term goal is to comprehensively understand the function and regulation of T3SS and use this knowledge to develop agents to curb bacterial infection.
Comparative and functional genomics of Pseudomonas savastanoi pv. glycinea, and identification of new resistance sources of soybean against race 4 Soybean - (Glycine max (L.)), one of the world’s largest providers of protein and oil, is a major crop in the United States, which accounts for about 40% of the soybeans produced in the world. Bacterial blight, caused by Pseudomonas savastanoi pv. glycinea (Psg), is a common bacterial disease of soybean and occurs in most soybean grown areas. Yield losses due to bacterial blight disease of soybean estimated at 4 to 40% in the U.S. Genome link: http://www.ncbi.nlm.nih.gov/nuccore/320326756; http://www.ncbi.nlm.nih.gov/nuccore/320332072; and http://www.pseudomonas-syringae.org/
PLPA406 Phytobacteriology, two credit hours; second 8-weeks of spring semester
PLPA509 Molecular Biology of Plant Microbe Interactions, three credit hours, spring semester of even years
Lee, J. H., Sundin, G. W., and Zhao, Y. F. 2015. Identification of the HrpS binding site in the hrpL promoter and effect of the RpoN binding site of HrpS in regulation of the type III secretion system in Erwinia amylovora.Mol. Plant Pathol. DOI: 10.1111/mpp.12324.
Lee, J. H., and Zhao, Y. F. 2016. Integration host factor is required for RpoN-dependent hrpL gene expression and controls motility by positively regulating rsmB sRNA in Erwinia amylovora.Phytopathology 106:29-36.
Chatnaparat, T., Li, Z., Korban, S. S., and Zhao, Y. F. 2015. The bacterial alarmone (p)ppGpp is required for virulence and controls cell size and survival of Pseudomonas syringae on plants. Environ. Microbiol. 17:4253-4270.
Chatnaparat, T., Li, Z., Korban, S. S., and Zhao, Y. F. 2015. The stringent response mediated by (p)ppGpp is required for virulence of Pseudomonas syringae pv. tomato and its survival on tomato. Mol. Plant-Microbe Interact. 28:776-789.
Ancona, V., Lee, J. H., Chatnaparat, T., Oh, J., Hong, J., and Zhao, Y. F. 2015. The bacterial alarmone (p)ppGpp activates type III secretion system in Erwinia amylovora.J. Bacteriol. 197:1433-1443.
Ancona, V., Chatnaparat, T., and Zhao, Y. F. 2015. Conserved aspartate and lysine residues of RcsB are required for amylovoran biosynthesis, virulence, and DNA binding in Erwinia amylovora.Mol. Gen. Genomics 290: 1265-1276.
Li, W., Anocona, V., and Zhao, Y. F. 2014. Co-regulation of polysaccharide production, motility, and expression of type III secretion genes by EnvZ/OmpR and GrrS/GrrA systems in Erwinia amylovora. Mol. Gen. Genomics 289:63-75.
Anocona, V., Li, W., and Zhao, Y. F. 2014. Alternative sigma factor RpoN and its modulation protein YhbH are indenpensible for Erwinia amylovora virulence. Mol. Plant Pathol. 15:58-66.
Yang, F., Korban, S.S., Pusey, P. L., Elofsson, M., Sundin, G. W., and Zhao, Y. F. 2014. Small molecule inhibitors suppress the expression of both type III secretion and amylovoran biosynthesis genes in Erwinia amylovora. Mol. Plant Pathol. 15:44-57.
Metelev, M., Serebryakova, M., Ghilarov, D., Zhao, Y. F., Severinov, K. 2013. Structure of microcin-B-like compounds produced by Pseudomonas syringae and species specificity of their antibacterial action. J. Bacteriol. 195: 4129-4137.
Khan, A. M., Zhao, Y. F., and Korban, S. S. 2013. Identification of genetic loci associated with fire blight resistance in Malus through combined use of QTL and association mapping. Physiol. Plantarum 148:344-353.
Wu, X., Vellaichamy, A., Wang, D., Zamdborg, L., Kelleher, N. L., Huber, S. C. and Zhao, Y. F. 2013. Differential lysine acetylation profiles of Erwinia amylovora strains revealed by proteomics. J. Proteomics 79:60-71.
Khan, A. M., Han, Y., Zhao, Y. F., Troggio, M., and Korban, S. S. 2012. A multi-population consensus genetic map reveals inconsistent marker order among maps likely attributed to structural variations in the apple genome. PLoS ONE 7:e47864.
Wang, D., Korban, S. S., and Pusey, L., and Zhao, Y. F. 2012. AmyR is a novel negative regulator of amylovoran production in Erwinia amylovora. PLoS ONE 7: e45038.
Kim, I., Pusey, L., Zhao, Y. F., Korban, S. S., Choi, H., and Kim, K. 2012. Microencapsulation and controlled release of Pantoea agglomerans E325 for biocontrol of fire blight disease of apple. J. Controlled Release 161:109-115.
Khan, M. A., Han, Y., Zhao, Y. F., and Korban, S. S. 2012. A high-throughput apple SNP genotyping platform using the GoldenGate Assay. Gene 494: 196-201.
Khan, M.A., Zhao, Y.F., and Korban, S.S. 2012. Molecular mechanisms of pathogenesis and resistance to the bacterial pathogen Erwinia amylovora, causal agent of fire blight disease in Rosaceae. Plant Mol. Biol. Rep. 30:247-260. (invited review)
McNally, R., Toth, I., Cock, P., Pritchard, L., Hedley, P., Morris, R., Zhao, Y. F., and Sundin, G. W. 2012. Genetic characterization of the HrpL regulon of the fire blight pathogen Erwinia amylovora reveals novel virulence factors. Mol. Plant Pathol. 13:160-173.
Wang, D. P., Qi, M. S., Calla, B., Korban, S. S., Clough, S. J., Cock, P., Sundin, G. W., Toth, I., and Zhao, Y. F. 2012. Genome-wide identification of genes regulated by the Rcs phosphorelay system in Erwinia amylovora. Mol. Plant-Microbe Interact. 25:6-17.
Wang, D. P., Calla, B., Vimolmangkang, S., Wu, X., Korban, S. S., Huber, S. C., Clough, S. J., and Zhao, Y. F. 2011. The orphan gene ybjN conveys pleiotropic effects on multicellular behavior and survival of Escherichia coli. PLoS ONE 6:e25293.(DOI:10.1371/journal.pone.0025293)
Sarowar, S., Zhao, Y. F., Guerra, R., Ali, S., Zheng, D., Wang, D. P., and Korban, S. S. 2011. Expression profiles of differentially regulated genes during early stages of apple flower infection with Erwinia amylovora. J. Exp. Bot. 62:4851-4861.
Zhao, Y. F., and Qi, M. S. 2011. Comparative genomics of Erwinia amylovora and related Erwinia species - what do we learn? Genes 2: 627-639 -- Special Issue [Genes and Genomes of Plant Pathogenic Bacteria]. (Invited review)
Wang, D., Korban, S. S., Pusey, L., and Zhao, Y. F. 2011. Characterization of the RcsC sensor kinase from Erwinia amylovora and other enterobacteria. Phytopathology 101:710-717.
Oh, M., Wang, X., Wu, X., Zhao, Y. F., Clouse, S. D., and Huber, S. C. 2010. Phosphorylation of Tyr-610 in the receptor kinase BAK1 plays a role in brassinosteroid signaling and affects basal defense gene expression. Proc. Natl. Acad. Sci. USA. 107: 17827-17832.
Di, C., Zhang, Q., Li. M., Hartman, G., and Zhao, Y. F. 2010. Image processing methods for quantitatively detecting soybean rust from multispectral images. Biosystems Engineering 107:186-193.
Nakka, S., Qi, M., and Zhao, Y. F. 2010. The Erwinia amylovora PhoPQ system is involved in resistance to antimicrobial peptide and suppresses gene expression of two novel type III secretion systems. Microbiol. Res. 165:665-673.
Qi, M., Sun, F., Caetano-Anolles, G., and Zhao, Y. F. 2010. Comparative genomic and phylogenetic analyses reveal the evolution of core two-component signal transduction systems in enterobacteria. J. Mol. Evol. 70: 167-180.
Wang, D., Korban, S. S., and Zhao, Y. F. 2010. Molecular signature of differential virulence in natural isolates of Erwinia amylovora. Phytopathology 100:192-198.
Nakka, S., Qi, M., and Zhao, Y. F. 2010. The PmrAB system in Erwinia amylovora renders the pathogen more susceptible to polymyxin B and more resistance to excess iron. Res. Microbiol. 161:153-157.
Koczan, J. M., McGrath, M., Zhao, Y. F., and Sundin, G. W. 2009. The contribution of the exopolysaccharide amylovoran and levan to biofilm formation: Implication in pathogenicity. Phytopathology 99:1237-1244.
Zhao, Y. F., Wang, D., Nakka, S., Sundin, G. W., and Korban, S. S. 2009. Systems level analysis of two-component signal transduction systems in Erwinia amylovora: Role in virulence, regulation of amylovoran biosynthesis and swarming motility. BMC Genomics. 10:245. (http://www.biomedcentral.com/content/pdf/1471-2164-10-245.pdf)
Li, X., Nie, J., Ward, L., Madani, M., Hsiang, T., Zhao, Y. F., De Boer, S. 2009. Comparative genomics-guided loop-mediated isothermal amplification for characterization of Pseudomonas syringae pv. phaseolicola. J. Appl. Microbiol. 107:717-726.
Zhao, Y. F., Sundin, G. W., and Wang, D. P. 2009. Construction and analysis of pathogenicity island deletion mutants in Erwinia amylovora. Can. J. Microbiol. 55:457-464.
Di, C., Zhang, Q., Li. M., Zhao, Y. F., and Hartman, G. 2009. Detection of soybean rust using a multispectral image sensor. Sensing and Instrumentation for Food Quality and Safety. 3:49-56.
Wang, D. P., Korban, S. S., and Zhao, Y. F. 2009. The Rcs phosphorelay system is essential for pathogenicity in Erwinia amylovora. Mol. Plant Pathol. 10:277-290.
Berry, M., McGhee, G. C., Zhao, Y. F., and Sundin, G. W. 2009. Effect of a waaL mutation on lipopolysaccharide composition, oxidative stress survival, and virulence in Erwinia amylovora. FEMS Microbiol. Lett. 291:80-87.
Wise, K. A., Zhao, Y. F., and Bradley, C. A. 2008. First report of pink seed of pea caused by Erwinia rhapontici in North Dakota. Plant Dis. 92:315
Perez-Martinez, I., Zhao, Y. F., Murillo, J., Sundin, G. W., and Ramos, C. 2008. Global genomic analysis of Pseudomonas savastanoi pv. savastanoi plasmids. J. Bacteriol. 190:625-635.
Ma, Z., Smith, J. J., Zhao, Y. F., Jackson, R., Arnold, D., Murillo, J., and Sundin, G. W. 2007. Phylogenetic analysis of the pPT23A plasmid family of Pseudomonas syringae. Appl. Environ. Microbiol. 73:1287-1295.
Triplett, L., Zhao, Y. F., and Sundin, G. W. 2006. Genetic differences among blight-causing Erwinia species with differing host specificities identified by suppression subtractive hybridization. Appl. Environ. Microbiol. 72:7359-7364.
Zhao, Y. F., He, S. Y., and Sundin, G. W. 2006. The Erwinia amylovoraavrRpt2EA gene contributes to virulence on pear and AvrRpt2EA is recognized by Arabidopsis RPS2 when expressed in Pseudomonas syringae. Mol. Plant-Microbe Interact.9:644-654.
Zhao, Y. F., Blumer, S. E., and Sundin, G. W. 2005. Identification of Erwinia amylovora genes induced during infection of immature pear tissue. J. Bacteriol. 187:8088-8103.
Zhao, Y.F., Ma, Z., and Sundin, G. W. 2005. Comparative genomic analysis of the pPT23A plasmid family of Pseudomonas syringae. J. Bacteriol. 187:2113-2126.
Sundin, G. W., Mayfield, C. T., Zhao, Y. F., Gunasekera, T. S., Foster, G. L., and Ullrich, M. S. 2004. Complete nucleotide sequence and analysis of pPSR1 (72,601 bp), a pPT23A family plasmid from Pseudomonas syringae pv. syringae A2. Mol. Genet. Genomics. 270:462-475.
Li, L, Zhao, Y. F., McCaig, B.,Wingerd, B., Wang, J., Whalon, M., Pichersky, E., and Howe, G. A. 2004. The Tomato homolog of CORONATINE-INSENSITIVE1 is required for the maternal control of seed maturation, jasmonate-signaled defense responses, and glandular trichome development. Plant Cell 16:126-143.
Zhao, Y. F., Thilmony, R., Bender, C. L., Schaller, A., He, S. Y., and Howe, G. A. 2003.Virulence systems of Pseudomonas syringae pv. tomato promotes bacterial speck disease in tomato by targeting the jasmonate signaling pathway. Plant J. 36:485-499.
Alarcon-Chaidez, F. J., Lisa, K., Zhao, Y. F., and Bender, C. L. 2003. RpoN (σ54) is required for plasmid-encoded coronatine biosynthesis in Pseudomonas syringae. Plasmid 49:106-117.
Zhao, Y. F., Damicone, J. P., and Bender, C. 2002. Detection, survival, and sources of inoculum for bacterial diseases of leafy crucifers in Oklahoma. Plant Dis. 86:883-888.
Zhao, Y. F., Jones, W. T., Sutherland, P., Palmer, D. A., Mitchell, R. E., Reynolds, P. H. S., Damicone, J. P., and Bender, C. L. 2001. Detection of the phytotoxin coronatine by ELISA and immunolocalization in infected plant tissue. Physiol. Mol. Plant Path. 58:247-258.
Jones, W. T., Harvey, D., Zhao, Y. F., Mitchell, R., Bender, C. L., and Reynolds, P. H. S. 2001. Monoclonal antibody-based immunoassays for the phytotoxin coronatine. Food Agric. Immunol. 13:19-32.
Zhao, Y. F., Damicone, J. P., Demezas, D., Rangaswamy, V., and Bender, C. 2000. Bacterial leaf spot of leafy crucifers in Oklahoma caused by Pseudomonas syringae pv. maculicola. Plant Dis. 84:1015-1020.
Zhao, Y. F., Damicone, J. P., Demezas, D., and Bender, C. 2000. Bacterial leaf spot diseases of leafy crucifers in Oklahoma caused by pathovars of Xanthomonas campestris. Plant Dis. 84:1008-1014.
McNally, R. Zhao, Y. F., and Sundin G. W. 2015. Towards understanding fire blight: virulence mechanisms and their regulation in Erwinia amylovora. In "Plant pathogenic bacteria: From basic research through systems biology to disease control." Jesus Murillo et al. (Eds). Horizon Press.
Ancona, V., and Zhao, Y. F. 2015. Microbial Associated Molecular Patterns. In "Virulence Mechanisms of Plant Pathogenic Bacteria," APS Press.
Zhao, Y. F. 2014. Genomics of Erwinia amylovora and related species associated with pome fruit trees. In "Genomics of Plant-Associated Bacteria." Dennis Gross, Ann Lichens-Park, and Chittaranjan Kole (eds). Springer.